Not just knowledge, but the knowledge of good and evil. Which includes the experience of both good and evil, as well as their consequences. Adam and Eve experienced the consequences of disobedience (evil).
As regards the Uncertainty Principle (from Quantum Physics) it is more than likely that it was already a part of the universe before the Fall (as were all Natural Laws).
"The uncertainty principle is certainly one of the most famous aspects of quantum mechanics. It has often been regarded as the most distinctive feature in which quantum mechanics differs from classical theories of the physical world. Roughly speaking, the uncertainty principle (for position and momentum) states that one cannot assign exact simultaneous values to the position and momentum of a physical system. Rather, these quantities can only be determined with some characteristic “uncertainties” that cannot become arbitrarily small simultaneously."
https://plato.stanford.edu/entries/qt-uncertainty/